![]() ![]() The groom and his men would avail themselves of every treatment Como and his assistants had to offer. He did especially well when one of his customers would marry. They were well-paid, did not mind spending money on themselves, and enjoyed Como's song renditions. His customers worked mainly at the nearby steel mills. Como had so much work after moving to the coffee house, he had to hire two barbers to help with it. One of Como's regular customers at the barber shop owned a Greek coffee house that included a barber shop area, and asked Como whether he would like to take over that portion of his shop. Practicing on his father, he mastered the skills well enough to have his own shop at age 14. ĭespite his musical ability, Como's primary ambition was to become the best barber in Canonsburg. Como and his brothers became the support of the household. When Como was 14, his father became unable to work because of a severe heart condition. His father told him he was entitled to make a mistake and that he hoped his son would never do anything worse than this. He managed to tell his father what had happened to the money his family depended on. Filled with shame, he locked himself in his room and did not come out until hunger got the better of him. Also around this time, Como lost his week's wages in a dice game. By age 13, he had graduated to having his own chair in the Fragapane barber shop, although he stood on a box to tend to his customers. Ĭomo started helping his family at age 10, working before and after school in Steve Fragapane's barber shop for 50¢ a week. Como was a member of the Canonsburg Italian Band along with bandleader Stan Vinton, who was the father of singer Bobby Vinton, and often a customer at Como's barber shop. He showed more musical talent in his teenaged years as a trombone player in the town's brass band, playing guitar, singing at weddings, and as an organist at church. In a rare 1957 interview, Como's mother, Lucia, described how her young son also took on other jobs to pay for more music lessons Como learned to play many different instruments, but never had a voice lesson. Pietro, a mill hand and an amateur baritone, had all his children attend music lessons even if he could barely afford them. The family had a second-hand organ his father had bought for $3 as soon as Como was able to toddle, he would head to the instrument, pump the bellows, and play music he had heard. ![]() ![]() He did not begin speaking English until he entered school, since the Comos spoke Italian at home. He was the seventh of 13 children and the first American-born child of Pietro Como (1877-1945) and Lucia Travaglini (1883-1961), who both emigrated to the US in 1910 from the Abruzzese town of Palena, Italy. Early years Ĭomo was born in Canonsburg, Pennsylvania, about 20 miles (32 km) southwest of Pittsburgh. He has the distinction of having three stars on the Hollywood Walk of Fame for his work in radio, television, and music. Posthumously, Como received the Grammy Lifetime Achievement Award in 2002 and was inducted into the Long Island Music Hall of Fame in 2006. He received a Kennedy Center Honor in 1987 and was inducted into the Academy of Television Arts & Sciences Hall of Fame in 1990. He also shared a Peabody Award with good friend Jackie Gleason in 1956. ![]() An example to all." Ĭomo received five Emmys from 1955 to 1959, and a Christopher Award in 1956. In the official RCA Records Billboard magazine memorial, his life was summed up in these few words: "50 years of music and a life well lived. His weekly television shows and seasonal specials were broadcast throughout the world. C.", as he was nicknamed, sold millions of records and pioneered a weekly musical variety television show. He recorded primarily vocal pop and was renowned for recordings in the intimate, easy-listening genre pioneered by multimedia star Bing Crosby. During a career spanning more than half a century, he recorded exclusively for RCA Victor for 44 years, after signing with the label in 1943. Pierino Ronald " Perry" Como ( / ˈ k oʊ m oʊ/ – May 12, 2001) was an American singer, actor, and television personality. ![]()
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![]() If you’re a fan of history then take a tour to see how the Habsburg royals lived. Take a Tour of Vienna’s Magnificent Imperial Palaces:.And there’s plenty of things to do in Vienna in the winter – outdoors and indoors. Furthermore, the city is less crowded as compared to summer. ![]() Still, there are plenty of activities you can do to enjoy the winter in Vienna. The local authorities clear the roads and footpaths very quickly to ensure smooth travel for locals and tourists. You can expect frequent snowfall during these months. Day temperatures below zero are not uncommon. From December to February are the coldest months where the temperature hardly exceeds 0☌. Vienna enjoys a dry continental climate with cold winters and hot summers.
![]() ![]() Ribozymes were first discovered in viral-like RNA pathogens of plants (hammerhead and hairpin) or of humans (HDV), and sporadically in metazoan genomes. They catalyze site-specific cleavage of RNA, and in some cases, the reverse ligation reaction via a concerted general acid–base mechanism. Nucleolytic ribozymes (hereafter referred to as ribozymes for short) are small RNAs (less than 200 nt) that function independently of proteins, of which nine classes are known to exist (hammerhead, hairpin, Varkud satellite, hepatitis delta virus (HDV), glmS, twister, twister-sister, pistol and hatchet) each with distinctive folding patterns. Distinct classes of RNA catalyse biochemical reactions (ribozymes), although the large subunit rRNA and RNaseP ribozymes common to all organisms require protein partners to function. The molecular functions of ribonucleic acid (RNA) are diverse and essential in all organisms. Expected size of RNaseJ1-6xHis protein is 63 kDa. Lane M indicates Kaledioscope™ prestained protein ladder (Bio-Rad). Lane 1 and 4 indicates protein lysates from pEGFP-hDHFR_iRJ1, lane 2 and 5 indicates protein lysates from pRzI_iRJ1, lane 3 and 6 indicates protein lysates from pRzII_iRJ1, lane 7, 10, 13 and 18 indicates protein lysates from iEGFP-hDHFR_iRJ1, lane 8, 11, 16 and 21 indicates protein lysates from iRzI_iRJ1, lane 9, 12, 17 and 22 indicates protein lysates from iRzII_iRJ1, lane 14 and 19 indicates protein lysates from i glmS_iRJ1, lane 15 and 20 indicates protein lysates from iM9_iRJ1, respectively. (A and C) Total protein-stained membrane (B and D) Immunodetection of RNaseJ1-6xHis using Anti-6X His IgG, CF™680 (Sigma-Aldrich, Merck KGaA, Germany). Western blot analysis of RNaseJ1-6xHis protein in single and double integrants All protein samples were extracted from integrants cultured in the absence (lane 1–3, 7–9, and 13–17) or presence of 0.0012% (w/v) arabinose (lane 4–6, 10–12, and 18–22) harvested after 8 h induction time. P-values from single value two-tailed t-tests comparing group mean to 1 of EGFP-hDHFR_33, RzI_33, RzII_33, glmS_33, and M9_33 are 0.8902, 0.9995, 0.9644, 0.8562, and 0.8483 respectively.Īdditional file 5: Figure S5. Dots indicate relative fluorescence data from individual experiments, and median values are indicated by the bold black line. Box plots show relative fluorescence data distribution. ![]() (B) Fluorescence intensity of i33 double integrants cultured in 0.0012% (w/v) arabinose relative to untreated control. Points represent the mean of 4–12 experiments and error bars represent 95% confidence intervals. Data are shown for each cell type grown in the presence or absence of 0.0012% (w/v) arabinose. OD 600 was measured every hour from 0 to 8 h cultivation time and data were plotted using the Growthcurve package in R software. (A) Growth analysis of double integrants ΔarsB::EGFP-hDHFR ΔlacZ::33 (iEGFP-hDHFR_i33), ΔarsB::RzIEGFP-hDHFR ΔlacZ::33 (iRzI_i33), ΔarsB::RzIIEGFP-hDHFR ΔlacZ::33 (iRzII_i33), ΔarsB:: glmSEGFP-hDHFR ΔlacZ::33 (i glmS_i33), and ΔarsB::M9EGFP-hDHFR ΔlacZ::33 (iM9_i33). Growth analysis and fluorescence intensity of iBAD33 double integrants. The Creative Commons Public Domain Dedication waiver ( ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.Īdditional file 4: Figure S4. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. ![]() ![]() This means that the Proxima Centurans don't know who won the last US Election, or that there are going to be new Star Wars movies. The closest star, Proxima Centauri, is more than 4.2 light-years away. If we had a telescope strong enough to watch the close encounter, we'd be looking at events that happened 4.6 hours ago. When NASA's New Horizons spacecraft reaches Pluto next year, it'll be 4.6 light hours away. When we look out into space, we’re also looking back into time. ![]() Curiosity landed minutes earlier, and we had to wait for the radio signals to reach us, since they travel at the speed of light. When we were watching live coverage of NASA's Curiosity Rover landing on Mars, it wasn't live. On average, Mars is about 14 light minutes away from Earth. You're not seeing the sun as it is, but how it looked more than 8 minutes ago. Our sun is 8 minutes and 20 seconds away at the speed of light. Even trying to communicate with someone on the moon would be frustrating as you'd experience a delay each time you talked. ![]() If there was a large explosion on the moon of a secret Nazi base, you wouldn't see it for just over a second. Light takes about 1.28 seconds to get from the moon to the Earth. The distance to the moon is, on average, about 384,000 km. And the further things are, the further back in time you're looking. When you look around you, you're not seeing the world as it is, you're seeing the world as it was, a fraction of a second ago. You're always seeing your loved ones 3.336 nanoseconds into the past. The light from your friend's face took about 3.336 nanoseconds to reach you. Let's say that you're talking with a friend who's about a meter away. “The universe just wasn’t building big galaxies at that point,” Robertson says. Astronomers don’t yet know exactly when the first dust and gas began to accrete into galactic clouds, and stars then accreted within them, but the newly measured quartet emerged very early in that process. The explanation for that is a straightforward one. “These galaxies are 100 million to a billion times the mass of the sun in stars.” “The Milky Way is a few tens of billions of times the mass of the sun in stars,” says Robertson. The galaxies are impressive by dint their age, but not by dint of their size and mass. Read more: Scientists Learn More About the History of Stars in Latest Webb Telescope Images “These are well beyond what we could have imagined finding before ,” said Robertson in a statement accompanying the release of the paper. Those numbers put the galaxies on a continuum from about 450 million years after the Big Bang-13.35 billion years ago-to 325 million years after the Big Band, or 13.475 billion years ago. “For most galaxies,” says Robertson, “the highest red shifts we had spectra for were at six, seven, or eight.”Īgainst those relatively modest standards, the four galaxies Robertson’s team imaged blew the doors off the old record, weighing in with red shifts of 10.38, 11.58, 12.63, and 13.2. The cosmic background radiation, a burst of universe-wide energy that was released just 380,000 years or so after the Big Bang has a red shift of about 1,100. The higher the number, the greater the movement of an object away from Earth. An object like Jupiter, which is pretty much stationary in the sky relative to Earth, has a red shift of zero. Instead, it is just a number that indicates how stretched the wavelength of the light is. Red shift is a tricky thing to measure, because it has no particular units like inches or nanometers. The redder an object appears, the more distant and old it is in our still-expanding universe. ![]() As objects move away from us, the wavelength is stretched, shifting it toward the red end. As objects move toward us in space, the wavelength of light they emit is compressed, shifting it to the bluer end of the visible spectrum. ![]() Using two instruments aboard the Webb-the Near Infrared Camera (NIRCam) and the Near Infrared Spectrograph (NIRSpec)-Robertson and his colleagues focused on four galaxies that appeared especially small, faint, and distant, studying what is known as their red shift. ![]() ![]() ![]() Driven by his passion for sound for picture, Stefan founded Decibel Peak, a platform designed to empower and support emerging sound professionals while contributing to the growth of the industry. Today, Stefan utilizes his extensive expertise to record production sound and lead the audio post-production process for a variety of projects in the TV, Film, and New Media industry. His journey into sound production began at the young age of 16, where he initially produced music that went on to feature on local television. In this video, we’ll take an in-depth look at the features of the Logic Pro X compressor, as well as the 7 different compression types that are modeled after analog hardware units. Stefan is a highly proficient sound professional who specializes in sound for picture. Thankfully, Logic Pro X includes one of the best stock compression plugins around and it’s extremely versatile for many different tasks. TEORIA: 00:00 - 02:25PRATICA: 02:33 - 09:11Che cosè il compressore e come viene utilizzatoIn questo video utilizzeremo il compressore di Logic per praticit. What’s your biggest difficulty when it comes to mixing and mastering? The speaker monitors are STRONGLY recommended, but I lived without them for many years.ĭo you have any specific questions about mixing and mastering? Let us know in the comments and feel free to share your experience with us! Other than that, you’ll just need an excellent pair of headphones (or two). I also use MANY different devices to test my mixes on.Įverything else is subjective, but those are the essential plugins you’ll be needing for both mixing and mastering. ![]() Personally, I just work with 2 pairs of headphones and one pair of speaker monitors. That being said, it really depends on how much money you want to put down (and how much space you have). Some of the old-school mixing and mastering engineers still swear by rack mount effects processors (instead of using plugins). Logic Pro X is the most advanced version of Logic ever. With the recent popularization of spatial audio (which you can read about HERE), a special type of speaker referred to as a “soundbar” could also be a good investment. Now, the sky’s the limit when it comes to equipment you COULD use for mixing and mastering. While these templates are 100 free, some websites may require you to register on their website or subscribe to their mailing list. Templates are a great way to learn new skills and stay up to speed with the most current trends in sound design. Of course, that’s when the acoustics of your room/studio come into play…Ĭan you work without speaker monitors (if you really don’t have a choice)? Absolutely, that’s why I’d personally prioritize headphones. A new list of the latest finds in FREE templates, presets, plugins and more for Logic Pro X. Speaker monitors are essential because headphones can only go so far in regards to recreating the sound of a room (referred to as “soundstage”). Headphones are the most important piece of equipment (if you ask me). ![]() |